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KMID : 0357419950250020155
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Korean journal of Virology
1995 Volume.25 No. 2 p.155 ~ p.162
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Expression of HPV16 E6 Gene Isolated from Korean Cervicla Carcinoma Tissues in E. coli
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이영희
진승원/윤희식/박병철/임종석/이희구/정현호/최인성/박순희
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Abstract
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Human papillomaviruses, especially type 16 and 18, have been reported to be closely associated with human cervical lesions and carcinoma, One of the open reading frames, E6 has been identified as a transforming gene. We have isolated the HPV16 E6
oncogene from Korean cervical carcinoma tissues, subcloned into an expression plasmid pET-3a, and produced 16E6 proteins in E. coli under the control of T7 promoter. Recombinat E6 protein was successfully produced in high level upto 30% of total
proteins and specifically recognized by anti-16E6 polyclonal antibodies produced by rabbit immunized with TrpE-16E6 fusion protein. 16E6 protein was produced in insoluble form, Currently we are establishing purification procedure of solubilized
E6
protein.
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KEYWORD
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